HoloTrap: Interactive hologram design for multiple dynamic optical trapping

This work presents an application that generates just-in-time holograms to be displayed in a holographic optical tweezers setup, a technique which allows the manipulation of particles in the range from micrometers to nanometers. The software is written in Java, and implements the random binary masks method to generate the holograms. It allows customization of the parameters adapted to the experimental setup and the used hologram display, taking into account the specific characteristics of the device, such as the modulation or the presence of aberrations. The performance of the software is evaluated, achieving 12 fps at full display resolution when run on a Pentium IV HT, 3.2 GHz computer, which allows real time interaction with the sample. Experimental results of the manipulation of microspheres using this program are provided.